Composite
PzraP-GFP

Part:BBa_K4385013:Design

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-03)


Zinc sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 907


Design Notes

The part consists of the basic parts GFP (BBa_E0040) and zinc-inducible promoter PzraP (BBa_K4385007). The initiation of the zinc-inducible promoter PzraP requires the support of the ZraSR two-component membrane associated sensor kinase system, which is present in the strain we used, E. coli XL-1 Blue.


Source

It comes from E. coli genomic DNA.

References